赛默飞培养箱311操作流程

习俊怡操作视频 h t y a p l 零一按 open 键打开泵头，直线安装软管，按 close 键关闭泵头， 安装杯体，调节转速。仪器运行时长按电源键开启压力报警指示灯亮。仪器运行时再次长按电源键，关闭压力报警指示灯灭。认识绿膜油性简品无需润膜。盖上红色护帽， 排出缓冲液，停止运行。打开红色护帽泄压公示品过滤启动运行。仪器转速稳定后倒转公示品屏盖上红色护帽过滤，停止运行。打开红色护帽泄压 冲洗。盖上红色护帽，排出缓冲液，打开红色护帽泄压。安装三角帽塞罐装培养机先 tsb 在 ftm 注意管路通断与切换，关闭软管夹， 剪断软管并插入杯体呼吸器上标记信息放入支架至规定温度培养。谢谢观看。

thermal scientific forma stereocult c o two incubator is a premier choice for use in a cell therapy setting learn how easy it is to clean and disinfect before beginning move all cultures to a different c o two incubator it is best to switch off the c o two incubator power during cleaning stereo called co two incubators are designed with shelves and supports that are easily removable without tools and the cts series features an electropolished stainless steel interior， which reduces microscopic structures to minimize areas where microorganisms could hide let's get started remove the shelves shelves supports in shelf rails and set aside for later cleaning or if you prefer send them to the autoclave for sterilization of course aside from regular maintenance cleaning and disinfection of the incubator any spilled growth media should be cleaned immediately to eliminate the chance that microorganisms could grow in that area use an approved disinfectant wipe containing ten percent or less quaternary ammonium or three percent hydrogen peroxide or a combination of one percent hydrogen peroxide zero point， eight， per acetic acid and less than ten percent acetic acid for example spork lends ready to use disinfectant wipes from stereos biosciences for all disinfectants follow the manufacturer's recommendations for use and dwell time and in all cases we recommend following with seventy percent ethanol or seventy percent isopropanol to remove the disinfectant residues the tables shown here lists recommended disinfectants gloves and eye protection should always be worn when handling chemical disinfectants in mid air fold the cloth in half， then fold in half again so it is folded in quarters hold the folded cloth between thumb and four fingers so that the fold is toward the surface to be cleaned and the edges are facing the palm of the hand there are many different types of service cleaners with different compositions and concentrations the level of risk varies with the chemical and concentration， but in every case it is important to check the safety data sheet and manufacturer's recommendations for you use starting at the furthest spot and at the top of the area to be cleaned wipe in a straight line toward you now refold the cloth to expose a new unused side and repeat the straight line wipe overlapping no more than ten to twenty percent of the previous straight line wipe at the end of each stroke lift the wipe completely and cleanly from the surface repeat this until the cloth has been used eight times and no side has been used more than once then discard the used cloth and take a new disposable cloth wipe continue until all surfaces have been cleaned and disinfected if a disinfectant other than seventy percent ethanol or seventy percent isopropanol was used repeat these steps with one of these disinfectants to remove any remaining residues starting with the back polasters carefully wipe each with the disinfectant allow the disinfectant to work for a few minutes then follow with seventy percent ethanol or seventy percent isopropanol then install the parts in the incubator chamber next carefully wipe each shelf with the disinfectant allow the disinfectant to work for a few minutes then follow with seventy percent ethanol or seventy percent isopropanol then install the shelves in the incubator chamber starting with the top inner corner wipe the door gaskets then wipe the inside of the glass door allow a few minutes for the disinfectant to work the length of this time interval depends on your disinfectant choice and the manufacturers recommend it then follow with seventy percent ethanol or seventy percent isopropanol and close the inner glass door following the same procedure starting at the top inner corner wipe the gasket and interior of the outer heated door close the door when complete when the interior cleaning and disinfection is complete be sure to clean and disinfect the exterior in the same way， the top of the incubator is too difficult to reach consider using a clean room up with disinfectant to reach these areas continue to wipe back to front top to bottom in a straight line motion overlapping no more than ten to twenty percent of the previous wipe as with all processes in a clean room setting and in accordance with iso one four six， four four dash thirteen all cleaning and disinfection practices must be validated for example use a wipe test or swab test following the procedure to document effectiveness of the process when complete switch on the power if desired initiate the one hundred forty degree celsius sterilization cycle at this time refer to the user manual for more information including steps to take before operating the sterilization cycle such as removal of the in chamber hepa filter and the sensors thank you for your interest in proper cleaning and disinfection of stereocalled co two incubators we hope this information is helpful and we wish you success in your process。

this instructional video will review methods for maintenance and feeding of stem scale cultures psc spheroids can be observed twenty four hours after the initiation of stemscale ps suspension cultures to feed stem scale psc medium suspended cultures we recommend a fifty percent medium replacement strategy to feed stem scale psc suspension cultures you will need complete stem scale medium and aco ii resistant shaker to perform the media of replacement swirl， the plate in a slow clockwise motion for spheroids to gather at the center of the well tilt the plate at a forty five degree angle and wait for five minutes for the spheroids to settle at the bottom be a gravity sedimentation once that spiroids have settled to the bottom of the well remove fifty percent of the spent medium from the top of the well to avoid aspirating the settled spiroids replace the medium with freshly prepared stem scale pse suspension medium without the white two seven six， three two compound for other culture vessel formats such as shaker flasks a similar method can be used to replace fifty percent of the medium without tilting the suspension culture vessel once the fresh medium has been added return the psc suspension cultures to the incubator and place onto the cot resistant orbital shaker with the same recommended settings as the spheroids continue to grow suspension cultures can be fed every other day using the same fifty percent media replacement strategy and setless feroids are ready to be passaged during this period wide two seven six， three two compounds should not be added to the media more instructional videos are available that cover the entire stem scale psc suspension workflow to find out more visit thermofisher com stem scale。

每个人来到这个世上都有一定的使命。泰瑞是赛莫飞苏州工厂的高级工程师， 每天早晨，他会与团队成员共同回顾昨天的业绩表现，并结合今天的计划定义出工作优先级及改善机会。 二零一零年，苏州工厂建成了亚洲第一条细胞工厂生产线。我们的生产车间为万级节电视。 我们严格控制温湿度、灰尘颗粒度以及陈将军水平。从原材料的选择、住宿、表面处理 到最终的焊接、包装，始终秉承匠人之心，并结合先进的设备完成高品质生产。我们的原材料源自欧洲，专为客户的细胞培养应用量身定制。 细胞工厂是纯物理焊接工艺加工而成的。在产品检测中，我们按照国际抽样标准 aql 来检测表面处理效果、焊接强度以及无热源水平。 同时，我们是唯一一家使用了四个细胞珠进行细胞培养检测的供应商。 carboy 主要应用于液体储存、其材质和耐高 高压灭菌。该生产线是亚太区唯一一条可生产五十升实验室用的大屏催速生产线。 在苏州工厂，每位员工都会定期接受安全培训、质量培训、岗位培训及 ppi 培训。 作为赛莫飞的一员，我们始终致力于为生物制药客户提供高质量的产品及服务，不断满足客户的需求并超越客户的期望。 赛摩飞苏州工厂成立于二零一二年，作为苏州工厂的负责人，卓越的产品质量一直是我们的 追求。苏州工厂已经通过了 so 九零零幺、 iso 幺四零零幺以及适用于医疗器械质量体系的 iso 幺三四八五的认证。 ppi 是我们的文化，已经融入到我们每个员工的日常生活中， 不仅帮助企业持续的改善流程，减少浪费，提高效率，而且提升了我们员工解决问题的能力。 in order to provide world class products and services we are adding additional production lines to meet directly growing needs of our production customers in asia this includes expanding the broader product range that we manufacturing in suzhou， such as the new easy fuel tool self factory sujo produces multiple products for multiple divisions of thermoficial specifically self factory systems and carboys for our bire production customers in asia pacific it's very important for thermoficiary to have a manufacturing presence in asia the bio production market is growing significantly in apec and socio allows us to significantly reduce lead times and offer more responsive and effective support for our customers in asia sujo is one of three manufacturing sites on three continents for self factory systems and one of sixteen globally for bio production allowing us to offer assurance of supply for business continuity planning。 慢病毒是一个很重要的一个原材料，而耐克的细胞工厂在我们的慢病毒生产工艺当中发挥了重要的作用，而前单核细胞工厂具有很好的一个使用的效果。而赛莫菲呢， 将来在基本口的培养机以及上营的设备工艺的开发过程当中，我们将会开展生物的合作。我们抗怀二零一一年成立之后，就跟咱们三门会这面就开展合作。 呃，我们主要是使用了咱们三分之这边的耐克细胞工厂以及培养机，咱们产品主要是应用我们前期的细胞培养以及病毒收获。通过这么多年咱们产品的使用效果，我们发现咱们产品质量还是非常稳定的，在产品的供货这方面也是非常及时，售后也非常棒。 suja provides the same high quality manufacturing environment locally， so we can meet customer process requirements globally and meet our mission to make the world healthier cleaner and safer。

how to culture pluripotent stem cells in suspension using the stem scale psc suspension medium， a scalable easy to use medium that supports robust expansion of pluripotent stem cells initiation of stem scale psc suspension cultures in this video you will learn how to culture plurry potent stem cells in suspension by using the gibtoe stem scale psc suspension medium as always use good cell culture practices and wear your personal protective equipment be sure to clean your cell culture hood and work surface by spraying and wiping them down with seventy percent alcohol stem scale psc medium suspended cultures can be initiated in various culture vessel， formats and sizes before you begin prepare the required number of suspension culture vessels ahead of dissociating your adhering cultures suspension culture vessels should be filled with complete stem scale psc suspension medium supplemented with ten micromolar， y two seven six， three two compound store the vessels prepared with stem scale pse medium suspended cultures in a thirty seven degrees celsius five percent cotu incubator for later use to initiate the stem scale psc medium suspended cultures you will need gibcot stem pro accutase cell dissociation reagent gibcote dpbs， no calcium， no magnesium yibcoat complete stem scale medium， y two seven six， three two compound and a cot resistant shaker start by aspirating the psc medium from your ed harring cultures next watch the adheritant culture by adding dpvs to the well aspirate the dpvs solution once the wash has been performed to associate the adherent pse cultures into a single cell suspension by adding stem pro accutates celled association reagent to each well incubate the cells for forty five minutes at thirty seven degrees celsius after four to five minutes remove the culture vessel from the incubator carefully trituate the stem pro accutate solution to to ensure the edit heron culture is associated into a single cell suspension neutralized with three milliliters of stem scale psc suspension medium her one milliliter of stem pro acutaze solution after collecting and centrifuging the single cell suspension re suspend the pellet in freshly prepared stem scale medium supplemented with ten micromolar of white two seven six， three two compound after determining the number of viable cells seed one hundred to one hundred fifty thousand cells per milliliter of medium in the suspended culture vessels prepared earlier gently mix the suspension culture vessels and place them on the cot resistant orbital shaker in the incubator make sure to adjust the speed settings of the cot resistant shaker to the recommended value for suspension cultures we recommend a speed of seventy rpm for six wall plates and one hundred twenty five milliliter shaker flask vessels more instructional videos are available that cover the entire stem scale psc suspension workflow to find out more visit thermofisher com stem scale。

passaging of stem scale psc suspension cultures passaging of the psc spheroids is recommended when they approach four hundred micromolar in size to avoid the formation of an aquatic core and loss of plurie potatsy when using stem scale psc suspension medium this generally occurs after four to five days of growth to passage the stem scale pse suspension cultures you will need stem pro acutate cell dissociation reagent complete stem scale medium， y， two seven six， three two compound， and a ceo， two resistant shaker when psc spheroids are rented to be passaged prepare the desired number of suspension culture vessels has described earlier to passage the psc spheroids start by swirling the culture vessel slowly in a circular motion to gather spheroids to the center of the well collect the spheroids by pipetting or pouring the spheroid containing medium into a fifty milliliter conical too watch the walls of the anti culture vessels with stem scale medium to collect any spheroids that may have been left behind collected spheroids should be centrifuged at two hundred pounds gravity for four minutes after centrifugation aspirate the spent stem scale psc suspension medium add the recommended volume of pre warmed stem pro accutates celled association reagent do not use a p one thousand pipette to church rate the spheroid pellet as this may negatively impact cell viability allow the spheroids to dissociate in a thirty seven degree celsius water for ten to fifteen minutes during the ten to fifteen minutes periodically mixed the spheroids by flicking or gently shaking the tube at intermittent intervals the cell suspension will become cloudy as more spyroids have dissociated in the single cells after ten to fifteen minutes of incubation in stem pro accutate cell dissociation reagent treat rate the cell suspension five to seven times using a p one thousand micropipette to further break up the spheroids into single cells or small clusters once the spheroids have completely dissociated at three milliliters of stem scale medium for one milliliter of dup co stem pro accutator to enactivate the dissociation reagent and mix by gentle inversion the single cell suspension should then be centrifuged and cells resuspended in fresh stem scale psc suspension medium supplements a button micromolar of y two， three， two similar to initiating the psc suspension cultures count and seed one hundred to one hundred fifty thousand cells per milliliter media in a new non tissue culture treated vessel before replacing the vessel on the cot resistant orbital shaker in the incubator more instructional videos are available that cover the entire stem scale psc suspension workflow find out more visit thermofisher com stem scale。